Molecular Docking Studies of Rifampicin – rpoB
                    complex: Repurposing Drug Design Implications for against Plasmodium
                        falciparum Malaria through a Computational Approach

Upasana Yadav; Jaya Pandey

doi:10.1055/a-1974-9028

Drug Research, Table of Contents

Drug Res (Stuttg) 2023; 73(03): 164-169
DOI: 10.1055/a-1974-9028

Original Article

Molecular Docking Studies of Rifampicin – rpoB complex: Repurposing Drug Design Implications for against Plasmodium falciparum Malaria through a Computational Approach

Upasana Yadav

¹Amity School of Applied Sciences Lucknow, Amity University Uttar Pradesh, Lucknow, India

,

Jaya Pandey

¹Amity School of Applied Sciences Lucknow, Amity University Uttar Pradesh, Lucknow, India

› Author Affiliations

Abstract

Malaria is one of the world’s most devastating diseases, infecting well over 300 million people annually and killing between 2 and 3 million worldwide. Increasing parasite resistance to many existing drugs is exacerbating disease. Resistance to commonly used malarial drugs is increasing the need to develop new drugs urgently. Due to the slow pace and substantial costs of new drug development, repurposing of old drugs which is recently increasingly becoming an attractive proposition of highly efficient and effective way of drug discovery led us to study the drug rifampicin for this purpose. The present paper aims to investigate the route of Plasmodium falciparum apicoplast-targeted proteins that putatively encode β subunits of RNA polymerase with an objective to develop an effective antimalarial drug. Homology searching for conserved binding site to the rifampicin drug and the functional analysis of rpoB gene were done. Multiple Sequence alignment analysis of rpoB was compared with that in E.coli – rpoB and M. tuberculosis – rpoB. Docking studies of Rifampicin – rpoB complex was also done for finding binding affinity. The results of computational studies showed that rifampicin is a potential drug for malaria.

Keywords

Antimalarial drugs - drug repurposing - rifampicin - parasite resistance - apicoplast - rpoB gene

Full Text

References

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